Alejandro San Martín PDF Print E-mail
Alejandro San Martín received his PhD. Degree in Molecular and Cell Biology from University Austral of Chile in 2014. He developed the first genetically-encoded FRET indicators for pyruvate (Pyronic) and lactate (Laconic). San Martín is an investigator at the Biology Department in the Center of Scientific Studies (CECs), Valdivia-Chile. His research focus on the development of optical tools to unravel cell energy metabolism.







PUBLICACIONES SELECCIONADAS
Arce-Molina R, Cortés-Molina F, Sandoval PY, Galaz A, Alegría K, Schirmeier S, Barros LF, San Martín A. (2019). A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate carrier MPC. bioRxiv 611806; doi: https://doi.org/10.1101/611806.

Contreras-Baeza Y, Ceballo S, Arce-Molina R, Sandoval PY, Alegría K, Barros LF, San Martín A. (2019). MitoToxy Assay: a novel cell-based method for the assessment of metabolic toxicity in a multiwell plate format using a lactate FRET nanosensor, Laconic. PLoS One. 2019 Oct 31;14(10):e0224527. doi: 10.1371/journal.pone.0224527. eCollection 2019.

Contreras-Baeza Y, Sandoval PY, Alarcón R, Galaz A, Cortés-Molina F, Alegría K, Baeza-Lehnert F, Arce-Molina R, Guequén A, Flores CA, San Martín A, Barros LF. (2019). MCT4 is a high affinity transporter capable of exporting lactate in high-lactate environments. J Biol Chem. 2019 Dec 27;294(52):20135-20147. doi: 10.1074/jbc.RA119.009093. Epub 2019 Nov 12.

San Martín, A., Arce-Molina, R., Galaz, A., Perez-Guerra, G., and Barros, L. F. (2017) Nanomolar nitric oxide concentrations quickly and reversibly modulate astrocytic energy metabolism. J. Biol. Chem. 292, 9432–9438.

Barros LF, San Martín A, Ruminot I, Sandoval PY, Fernández-Moncada I, Baeza-Lehnert F, Arce-Molina R, Contreras-Baeza Y, Cortés-Molina F, Galaz A, Alegría K. (2017). Near-critical GLUT1 and Neurodegeneration. J Neurosci Res. Feb 2. doi: 10.1002/jnr.23998.

Mächler P, Wyss MT, Elsayed M, Stobart J, Gutierrez R, von Faber-Castell A, Kaelin V, Zuend M, San Martín A, Romero-Gómez I, Baeza-Lehnert F, Lengacher S, Schneider BL, Aebischer P, Magistretti PJ, Barros LF and Weber B. (2016). In Vivo Evidence for a Lactate Gradient from Astrocytes to Neurons. Cell Metab. 2016 Jan 12;23(1):94-102. doi: 10.1016/j.cmet.2015.10.010.

Peetz J, Barros LF, San Martín A, and Becker HM. (2015). Functional interaction between bicarbonate transporters and carbonic anhydrase modulates lactate uptake into mouse cardiomyocytes. Pfflugers Arch. 2015 Jul;467(7):1469-80. doi: 10.1007/s00424-014-1594-z.

San Martín A, Sotelo-Hitschfeld T, Lerchundi R, Fernández-Moncada I, Ceballo S, Valdebenito R, Baeza-Lehnert Felipe, Alegría K, Contreras-Baeza Y, Garrido-Gerter P, Romero-Gómez I, and Barros L.F. (2014). Single-cell imaging tools for brain energy metabolism: a review. Neurophotonics. 2014 Jul;1(1):011004. doi: 10.1117/1.NPh.1.1.011004.

San Martín A., Ceballo S, Baeza-Lehnert F, Lerchundi R, Valdebenito R, Contreras-Baeza Y, Alegría K. and Barros LF. (2014). Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for Pyruvate. PLoS ONE 9(1): e85780. doi:10.1371/journal.pone.0085780 .

Barros LF, San Martín A, Sotelo-Hitshfeld T, Lerchundi R, Fernández-Moncada I, Ruminot I, Gutiérrez R, Valdebenito R, Ceballo S, Alegría K, Baeza-Lehnert F and Espinoza D (2013) Small is fast: astrocytic glucose and lactate metabolism at cellular resolution. Front. Cell. Neurosci. 7:27. doi: 10.3389/fncel.2013.00027.

San Martín, A., Ceballo, S., Ruminot, I., Lerchundi, R., Frommer, W.B., and Barros et al. (2013) A Genetically Encoded FRET Lactate Sensor and Its Use To Detect the Warburg Effect in Single Cancer Cells. PLoS ONE 8(2): e57712. doi:10.1371/journal.pone.0057712.

Bittner CX, Valdebenito R, Ruminot I, Loaiza A, Larenas V, Sotelo-Hitschfeld T, Moldenhauer H, San Martín A, Gutiérrez R, Zambrano M, Barros LF. Fast and reversible stimulation of astrocytic glycolysis by K+ and a delayed and persistent effect of glutamate. J Neurosci. 2011 Mar 23;31(12):4709-13.

Rauch MC, San Martín A, Ojeda D, Quezada C, Salas M, Cárcamo JG, Yañez AJ, Slebe JC, Claude A. Tacrolimus causes a blockage of protein secretion which reinforces its immunosuppressive activity and also explains some of its toxic side-effects. Transpl Immunol. 2009 Dec;22(1-2):72-81.

PATENT
San Martín, A., Galaz, A., & Barros, L.F. (2019) Patent application, Genetically encoded probe for detection and quantification of lactate/pyruvate ratio with high spatial and temporal resolution. Application number: PCT/IB2019/057089. Current Assignee: Centro de Estudios Cientificos.

San Martín, A., Ceballo, S. & Barros, L.F. (2013) Patent application, Genetically encoded probe for quantification of pyruvate concentration with high spatial and temporal resolution, and methods using the same for quantifying pyruvate transport, cellular pyruvate production and consumption, and mitochondrial pyruvate consumption in intact cells. Application number: PCT/IB2013/056766. Current Assignee: Centro de Estudios Cientificos.

San Martín, A., Ceballo, S., Frommer, W.B. & Barros, L.F. (2012) Patent application, Genetically encoded probe for quantification of lactate with high spatial and temporal resolution, and methods for quantifying metabolic rates and lactate transport using the same. International application number PCT/US12/33639. Current Assignee: Centro de Estudios Cientificos and Carnegie Institution of Washington.

MEETING PRESENTATIONS
A genetically-encoded FRET sensor for lactate: a new tool for cancer drug discovery. Center for Scientific Studies (CECs), Valdivia, University Austral of Chile, Valdivia, & Carnegie Institution of Wahington. Dubai, UAE, 2013. 5th International Conference on Drug Discovery & Therapy.
San Martín, A., Ceballo, S., Ruminot, I., Lerchundi, R., 3Frommer, W.B. & Barros, L.F.
New methods for the study of lactate metabolism with a genetically encoded FRET nanosensor. Center for Scientific Studies (CECs), Valdivia, University Austral of Chile, Valdivia, & Carnegie Institution of Wahington. Bacelona, Spain, 2012. Multidisciplinary approaches to quantify astrocyte neuron signaling.
San Martín, A., Ceballo, S., Ruminot, I., Lerchundi, R., Frommer, W.B. & Barros, L.F.
XXV Annual meeting of the chilean society for cell biology. LACONIC, a genetically encoded FRET nanosensor for lactate. Center for Scientific Studies (CECs), Valdivia, University Austral of Chile, Valdivia, Carnegie Institution of Wahington, 2011.
San Martín, A., Ceballo, S., Frommer, W.B.; Barros, L.F.
XXII Annual meeting of the chilean society for cell biology. “No catalitic region of hGBF-1 are essencial for his localitation and function in the secretory pathway”. Institute of Biochemistry, University Austral of Chile, Valdivia, Chile, 2008.
San Martín, A., Rauch, M.C. y Claude, A.
III International meeting of Farmacology y Therapéutic. Cuban society of Farmacology. Diabetogenic effect of immunosuppressive drugs FK506 by blocking insulin secretion in pancreatic cells. La Habana, Cuba, 2007.
Rauch, M.C., San Martín, A., Ojeda, D., Quezada, C., Salas, M., Cárcamo, J.G., Yánez, A.J., Slebe, J.C. y Claude, A
XX Annual meeting of the chilean society for cell biology. “Análisis de la función de hGBF-1 y hBIG-1 mediante el silenciamiento por siRNA y construcción de proteínas quiméricas”. Institute of Biochemistry, University Austral of Chile, Valdivia, Chile, 2006.
San Martín, A., Rosas, J., Rauch, M.C. y Claude, A.
CONTACT
Telephone : +56-63-234521
E-mail :lc.scec@ojelaa